Analysis Note

ControlMouse embryonic stem cell lysate

Application

Research CategoryEpigenetics & Nuclear Function

Research Sub CategoryChromatin Biology

Anti-Cbx7 Antibody is a Rabbit Polyclonal Antibody for detection of Chromobox protein homolog 7 (Cbx7) also known as hromobox protein homolog 7 & has been validated in WB & IP.

Western Blot (Snap i.d.™) Analysis: 2 µg/mL from a representative lot detected Cbx7 on 10 µg of human and mouse embryonic stem cell lysates.

Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in IP.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Chromobox protein homologue 7 (Cbx7) is a member of the mammalian chromobox family of proteins. As a component of the Polycomb repressive complex 1 (PRC1), Cbx7, is involved in the maintenance and prolongation of cultured epithelial cell lifespan, and has been shown to function independently of BMI-1 in the repression of INK4a/ARF, a known tumor suppressor locus. Cbx7 is also able to regulate cellular lifespan through maintaining CDKN2A repression. Studies have shown Cbx7 to be up-regulated in follicular lymphoma, suggesting potential as a human oncogene.

Immunogen

Epitope: C-terminus

GST-tagged recombinant protein corresponding to the C-terminus of mouse Cbx7.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blot in mouse embryonic stem cell lysate.

Western Blot Analysis: 0.25 µg/mL of this antibody detected Cbx7 on 10 µg of mouse embryonic stem cell lysate.

Specificity

This antibody recognizes Cbx7 at the C-terminus.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~21 kDa observed. A non-specific band at ~ 44 kDa and may be observed in some lysates.